DIA€βETACHIP TECHNOLOGY: artificial pancrea in closed loop

Applications

• rapid quality control of islets before transplantation, stem-cell differentiation protocols, stem-cell derived islets
• Real-time analysis of islet α-cells and β-cells, islets and their coupling, islets derived from iPSCs
• Organ-on-chip analysis to replace animal tests, to study drug effects, to study nutritional effects

Description

Islet cells plated on a Multi-Electrode Array (MEA) generate, in response to glucose or other nutrients and hormones, a flow of charges through ion channels near of the microelectrodes.

Two signals are observed: Slow potentials (SP) and action potentials (AP). They reflect single cell bahaviour (AP) and physiologically important intra-organ coupling (SP).

Electronic components provide on-line real-time processing of both types of signals, their direct analysis and scoring

Bénéfices

• Combination of biology and microelectronics offers a direct, easy and user-friendly approach to islet cell activity in physiological settings.

  It provides a versatile Organ-on-Chip approach.

   

• Value of DIAβETAchip technology :

  • evaluates single cells & islets;
  • offers user-friendly: automatic & multi-modal analysis; non-invasive & -
  • long-term analysis; analysis of intra-islet coupling;
  • repetitive testing of compounds;
  • data classification & storage
  • Research phase: Functional Analysis
  • Clinically oriented: Therapeutic efficacy of a compound: on the micro-organ